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SRX8701076: GSM4663990: Input_CTL; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 47.6M spots, 2.4G bases, 1.1Gb downloads

Submitted by: NCBI (GEO)
Study: Transcriptomic and epigenetic analysis of adipose tissue in Cushing Syndrome [human]
PRJNA588382 • SRP229337 • All experiments • All runs
show Abstracthide Abstract
Chronic glucocorticoids (GCs) exposure, resulting from endogenous Cushing Syndrome (CS) or prescribed GCs therapy, is associated with a high cardiometabolic burden. Moreover, previous studies have reported persistence of metabolic syndrome features after remission of hypercortisolism and in particular in visceral adipose tissue (VAT). Thus, in this study we want to analyze the transcriptomic alterations in VAT during active and cured CS and correlate these persistent gene expression changes with changes in histone modifications induced by GCs. Overall design: RNA-seq (18 samples) and ChIP-seq (26 samples) of human visceral adipose tissue. CS means human patients of Cushing and CTL controls.
Sample: Input_CTL
SAMN15496499 • SRS6978734 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: For RNAseq: RNA was extracted using RNeasy Lipid Tissue Mini kit (Qiagen). For ChIPseq: ChIP experiments were carried out following a standard protocol in the lab (Morey et al., Cell Stem Cell 2013). Libraries were prepared according to Illumina instructions.
Experiment attributes:
GEO Accession: GSM4663990
Links:
Runs: 1 run, 47.6M spots, 2.4G bases, 1.1Gb
Run# of Spots# of BasesSizePublished
SRR1218699547,592,3352.4G1.1Gb2021-09-04

ID:
11323299

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